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Pathology and Laboratory Medicine

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Educational Objective
Review efforts to detect SARS-CoV-2 in different types of clinical specimens

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JAMA

May 12, 2020

Research Letter

Article Information and Disclosures

Author Affiliations

¹National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China
²Beijing Ditan Hospital, Capital Medical University, Beijing, China
³Qingdao Municipal Center for Disease Control and Prevention, Qingdao, China

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An epidemic of respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in China and has spread to other countries.¹ Real-time reverse transcriptase–polymerase chain reaction (rRT-PCR) of nasopharyngeal swabs typically has been used to confirm the clinical diagnosis.² However, whether the virus can be detected in specimens from other sites, and therefore potentially transmitted in other ways than by respiratory droplets, is unknown.

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Article Information

Accepted for Publication: March 5, 2020.

Corresponding Author: Wenjie Tan, MD, PhD, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Rd, Changping District, Beijing 102206, China (tanwj@ivdc.chinacdc.cn).

Published Online: March 11, 2020. doi:10.1001/jama.2020.3786

Author Contributions: Drs Wang and Tan had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Drs Wang and Xu contributed equally.

Concept and design: Wang, Xu, Wu, Tan.

Acquisition, analysis, or interpretation of data: Xu, Gao, Lu, Han, Tan.

Drafting of the manuscript: Wang, Xu, Gao, Han, Tan.

Critical revision of the manuscript for important intellectual content: Xu, Lu, Wu, Tan.

Statistical analysis: Wang.

Obtained funding: Wang, Tan.

Administrative, technical, or material support: Xu, Gao, Lu, Han, Wu.

Supervision: Xu, Wu, Tan.

Conflict of Interest Disclosures: None reported.

Funding/Support: This work was supported by grants 2016YFD0500301 and 2020YFC0840900 from the National Key Research and Development Program of China and 2018ZX10101002 from the National Major Project for Control and Prevention of Infectious Disease in China.

Role of the Funder/Sponsor: The sponsors had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

Additional Contributions: We thank Gary Wong, PhD (CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China) for English revision of the manuscript, and clinical staff members in hospitals in Hubei Province, Shandong Province, and Beijing for collection of specimens. None of these individuals received compensation for their contributions.

References

Tan W , Zhao X , Ma X , et al. Notes from the field: a novel coronavirus genome identified in a cluster of pneumonia cases—Wuhan, China 2019-2020. China CDC Weekly. 2020;2(4):61-62.Google Scholar

Wang D , Hu B , Hu C , et al. Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus–infected pneumonia in Wuhan, China. JAMA. Published February 7, 2020. doi:10.1001/jama.2020.1585 PubMed Google Scholar

Zhang W , Du RH , Li B , et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg Microbes Infect. 2020;9(1):386-389. doi:10.1080/22221751.2020.1729071 PubMed Google Scholar Crossref

Pan Y , Zhang D , Yang P , Poon LLM , Wang Q . Viral load of SARS-CoV-2 in clinical samples. Lancet Infect Dis. Published online February 24, 2020. doi:10.1016/S1473-3099(20)30113-4 PubMed Google Scholar

Detection of SARS-CoV-2 in Different Types of Clinical Specimens

Preparing for the Most Critically Ill Patients With COVID-19: The Potential Role of ECMO

COVID-19 in Singapore—Experience and Critical Issues That Require Attention and Action

Presumed Asymptomatic Carrier Transmission of COVID-19

The Coronavirus Disease 2019 (COVID-19) Outbreak in China—Summary of a China CDC Report

RT-PCR Test Results in Patients Recovered From COVID-19

COVID-19—New Insights on a Rapidly Changing Epidemic

Response to COVID-19 in Taiwan: Big Data Analytics, New Technology, and Proactive Testing

Priorities for the US Health Community Responding to COVID-19

Care for Critically Ill Patients With COVID-19

Incorporating Test Characteristics Into SARS-CoV-2 Testing Policy—Sense and Sensitivity

Symptoms at COVID-19 Onset Among Health Care Workers in Washington State

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