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An epidemic of respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in China and has spread to other countries.1 Real-time reverse transcriptase–polymerase chain reaction (rRT-PCR) of nasopharyngeal swabs typically has been used to confirm the clinical diagnosis.2 However, whether the virus can be detected in specimens from other sites, and therefore potentially transmitted in other ways than by respiratory droplets, is unknown.
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Accepted for Publication: March 5, 2020.
Corresponding Author: Wenjie Tan, MD, PhD, National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Rd, Changping District, Beijing 102206, China (email@example.com).
Published Online: March 11, 2020. doi:10.1001/jama.2020.3786
Author Contributions: Drs Wang and Tan had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Drs Wang and Xu contributed equally.
Concept and design: Wang, Xu, Wu, Tan.
Acquisition, analysis, or interpretation of data: Xu, Gao, Lu, Han, Tan.
Drafting of the manuscript: Wang, Xu, Gao, Han, Tan.
Critical revision of the manuscript for important intellectual content: Xu, Lu, Wu, Tan.
Statistical analysis: Wang.
Obtained funding: Wang, Tan.
Administrative, technical, or material support: Xu, Gao, Lu, Han, Wu.
Supervision: Xu, Wu, Tan.
Conflict of Interest Disclosures: None reported.
Funding/Support: This work was supported by grants 2016YFD0500301 and 2020YFC0840900 from the National Key Research and Development Program of China and 2018ZX10101002 from the National Major Project for Control and Prevention of Infectious Disease in China.
Role of the Funder/Sponsor: The sponsors had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Additional Contributions: We thank Gary Wong, PhD (CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China) for English revision of the manuscript, and clinical staff members in hospitals in Hubei Province, Shandong Province, and Beijing for collection of specimens. None of these individuals received compensation for their contributions.
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