Question
What is the performance of commercially available gene expression profile tests in predicting cutaneous melanoma outcomes in patients with stage I or stage II melanoma?
Findings
In this systematic review and meta-analysis of 7 studies including 1450 participants, gene expression profile test performance varied significantly by disease stage in external validation studies and was better at identifying recurrence in patients with stage II disease than in those with stage I disease. Studies were rated as having moderate to high risk of bias, and the quality of evidence was assessed as low to very low.
Meaning
In patients with clinically localized melanoma, there was variation in gene expression profile test performance by disease stage, suggesting limited potential for clinical utility for patients with stage I melanoma.
Importance
The performance of prognostic gene expression profile (GEP) tests for cutaneous melanoma is poorly characterized.
Objective
To systematically assess the performance of commercially available GEP tests in patients with American Joint Committee on Cancer (AJCC) stage I or stage II disease.
Data Sources
For this systematic review and meta-analysis, comprehensive searches of PubMed/MEDLINE, Embase, and Web of Science were conducted on December 12, 2019, for English-language studies of humans without date restrictions.
Study Selection
Two reviewers identified GEP external validation studies of patients with localized melanoma. After exclusion criteria were applied, 7 studies (8%; 5 assessing DecisionDx-Melanoma and 2 assessing MelaGenix) were included.
Data Extraction and Synthesis
Data were extracted using an adaptation of the Checklist for Critical Appraisal and Data Extraction for Systematic Reviews of Prediction Modeling Studies (CHARMS-PF). When feasible, meta-analysis using random-effects models was performed. Risk of bias and level of evidence were assessed with the Quality in Prognosis Studies tool and an adaptation of Grading of Recommendations Assessment, Development, and Evaluation.
Main Outcomes and Measures
Proportion of patients with or without melanoma recurrence correctly classified by the GEP test as being at high or low risk.
Results
In the 7 included studies, a total of 1450 study participants contributed data (age and sex unknown). The performance of both GEP tests varied by AJCC stage. Of patients tested with DecisionDx-Melanoma, 623 had stage I disease (6 true-positive [TP], 15 false-negative, 61 false-positive, and 541 true-negative [TN] results) and 212 had stage II disease (59 TP, 13 FN, 78 FP, and 62 TN results). Among patients with recurrence, DecisionDx-Melanoma correctly classified 29% with stage I disease and 82% with stage II disease. Among patients without recurrence, the test correctly classified 90% with stage I disease and 44% with stage II disease. Of patients tested with MelaGenix, 88 had stage I disease (7 TP, 15 FN, 15 FP, and 51 TN results) and 245 had stage II disease (59 TP, 19 FN, 95 FP, and 72 TN results). Among patients with recurrence, MelaGenix correctly classified 32% with stage I disease and 76% with stage II disease. Among patients without recurrence, the test correctly classified 77% with stage I disease and 43% with stage II disease.
Conclusions and Relevance
The prognostic ability of GEP tests among patients with localized melanoma varied by AJCC stage and appeared to be poor at correctly identifying recurrence in patients with stage I disease, suggesting limited potential for clinical utility in these patients.